Giant-Size Captain America #1
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President Roosevelt institutes the top-secret program, "Project Rebirth." In a secret lab, scientist Dr. Erskine gives army 4F reject and volunteer Steve Rogers the "Super-soldier serum," which turns him into a virtual superman. But a Nazi spy who got past security murders Erskine before he can create an army of men like Rogers, and is killed when he runs headlong into some electrical machinery. Rogers becomes the costumed Captain America, as a symbol of freedom, and begins a career of smashing spy rings and the like. When Bucky discovers Steve's double-identity, he becomes Cap's partner.
Plot details indexed by the Grand Comics Database (CC BY-SA).